CD Skripsi
Biosintesis Dan Karaterisasi Nanopartikel Zno Menggunakan Ekstrak Daun Sirih Hijau (Piper Betle L.) Kering Untuk Apliksi Antibakteri
Bacterial resistance to antibiotics has become a global challenge, prompting the search for more effective and environmentally friendly antibacterial alternatives. This study aims to synthesize and characterize zinc oxide (ZnO) nanoparticles using a biosynthesis method with green betel leaf (Piper betel L.) extract as a reducing and stabilizing agent. The synthesis process was carried out by mixing betel leaf extract with zinc nitrate hexahydrate (Zn(NO₃)₂·6H₂O) solution, followed by the addition of NaOH until ZnO precipitate formed at pH variations of 6, 8, 10, and 12. Characterization results showed that ZnO nanoparticles exhibited an absorption peak in the 250–330 nm wavelength range with a band gap energy of 3.04–3.30 eV (UV-Vis analysis). XRD analysis confirmed a wurtzite hexagonal crystal structure with a 27 nm crystallite size. In comparison, SEM revealed spHerical morpHology with an average particle size of 465.53 nm. FTIR results identified the presence of Zn-O bonding at 541.50 cm⁻¹, along with organic residues from betel leaf extract. Antibacterial tests showed that ZnO nanoparticles inhibited the growth of StapHylococcus aureus (S. aureus) and Escherichia coli (E. coli), with the largest inhibition zone of 4.58 mm (S. aureus) at 0.3 g/mL and 3.4 mm (E. coli) at 0.1 g/mL. These findings suggest that ZnO nanoparticles have strong potential as natural antibacterial agents for biomedical and pHarmaceutical applications.
Keywords: Biosynthesis, ZnO nanoparticles, Piper betel L., antibacterial, UV-vis.
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