CD Skripsi
Pengaruh Naa, Bap Serta Kombinasi Naa Dan Bap Terhadap Pertumbuhan Eksplan Daun Sansevieria Ehrenbergii Yang Diperbanyak Secara In-Vitro
ABSTRACT
Sansevieria or mother-in-law's tongue is a succulent leaf ornamental plant
from the Agavaceae family, has beautiful and diverse shapes and colors.
Sansevieria became popular because it can absorb air pollutants. Compounds that
can be absorbed by Sansevieria are chloroform, formaldehyde, trichloroethylene,
benzene, and xylene. Sexual propagation is less efficient because growth and
budding are slow, while vegetative propagation by cuttings requires a large
number of parent plants. This issue can be overcome by the tissue culture method
with the addition of cytokinins and auxins on MS media. The purpose of this
study was to see the effect of the combination of NAA and BAP on the growth of
leaf explants of Sansevieria ehrenbergii and to obtain the best concentration for
the growth of leaf explants of Sansevieria ehrenbergii propagated in-vitro. The
study used a completely randomized design consisting of 8 treatments and 3
replications so that there were 24 experimental units. Parameters observed were
callus appearance, callus color, callus texture, callus weight, callus diameter, and
percentage of growth success. Data analysis uses analysis of variance and uses
DNMRT level of 5 % for further testing. The administration of several
concentrations of growth regulators in the form of NAA, BAP and the
combination of NAA and BAP affected the growth of leaf explants of Sansevieria
ehrenbergii. The treatment of giving NAA 2 mg.l-1 + BAP 1 mg.l-1 gave the best
effect on callus appearance, namely 75.75 HST, administration of NAA 1 mg.l-1 +
BAP 2 mg.l-1 gave the best effect on callus weight was 0.9033 g and callus
diameter was 19.133 mm and the administration of NAA 1 mg.l-1 +
BAP 1 mg.l-1 gave the best effect on the percentage of callus growth, which was
100%.
Keywords: NAA, BAP, tissue culture, Sansevieria, in-vitro
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