CD Skripsi

Penapisan Dan Uji Antagonis Jamur Endofit Tanaman Tomat (Lycopersicum Esculentum Mill.) Terhadap Fusarium Oxysporum Serta Identifikasinya Secara In Vitro

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ABSTRACT This research aims to select and test endophytic fungi isolates from tomato plants with high antagonistic activity against Fusarium oxysporum, the causal agent of fusarium wilt disease. The research was conducted from November 2020 to February 2021 at the Plant Disease Laboratory, Faculty of Agriculture, University of Riau. The research involved exploration methods (isolation and purification of endophytic fungi from tomato), observation (hypovirulence and identification isolates with high antagonistic activity, and experiment (antagonism, diameter and growth rate of higher antagonistic activity of endophytic). Data of antagonicity, diameter and growth rate of higher antagonicity of endophytic fungi were analyzed using analysis of variance. To compare the means among the treatments, DNMRT at level 5% were applied. The isolation of endophytic fungi from tomato plants yielded 18 isolates, consisting of 5 isolates from roots, 6 isolates from stems, and 7 isolates from leaves. The hypovirulence test of the 18 isolates resulted in 11 hypovirulent isolates (T2, T3, T4, T6, T7, T8, T10, T12, T15, T16, and T18) and 7 virulent isolates (T1, T5, T9, T11, T13, T14, and T17). Isolate T3 had the highest antagonistic activity (68,33%). The largest diameter and the highest growth rate of endophyte fungi was isolate T4 (90,00 mm and 32,33 mm.day-1). The identification results of the 6 isolates with high inhibitory activity against Fusarium oxysporum showed that isolate T3 belongs to the genus Fusarium, isolate T6 belongs to the genus Aspergillus, isolates T10, T12, and T16 belong to the genus Trichoderma, and isolate T4 remains unidentified.
Keywords : Endophytic fungi, Fusarium oxysporum, tomato, antagonistic acttvity

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1606123886

Penerbit

Pekanbaru : Universitas Riau – Fakultas Pertanian – Agroteknologi., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1606123886

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Agroteknologi

Info Detail Spesifik

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Pernyataan Tanggungjawab

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Versi lain/terkait

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