CD Skripsi
Isolasi Dan Uji Kemampuan Antibakteri Mikroba Endofit Dari Mangga Mempelam (Mangifera Laurina Bl.) Terhadap Bakteri Staphylococcus Aureus
ABSTRACT
Mempelam mango (Mangifera laurina Bl.) is a type of wild mango which has bioactive compounds as antibacterial. Endophytic microbes are microorganisms that live in plant tissues and have been coevolution for a long time, which have the same metabolite compounds as their host plants. Isolation of endophytic microbes from mempelam mango plants is expected to obtain endophytic microbes that produce the same antibacterial compounds as their host plants, especially against Staphylococcus aureus bacteria which have been resistant to several types of antibiotics. Isolation of endophytic microbes from leaves and bark of mempelam mango by direct method resulted in 18 isolates consisting of five endophytic bacteria, 13 endophytic fungi and no endophytic actinomycetes isolates. Antibacterial activity test using the agar diffusion method showed that all isolates were able to inhibit the growth of S. aureus with various inhibition zones. Endophytic bacterial isolates BDM3 and endophytic fungal isolates JDM5 were isolates with the largest average diameter of the inhibition zone, which were 13.13 mm and 14.75 mm, respectively.
Key findings: antibacterial, isolation, characterization, endophytic microbes,
S. aureus.
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