CD Skripsi
Isolasi Dan Seleksi Bakteri Penghasil Dekstran Dari Matoa (Pometia Pinnata) Dan Kopi (Coffea Liberica) Sebagai Upaya Mencari Penghantar Obat Berbasis Nano
ABSTRACT
Dextran is an exopolysaccharide in the form of homopolysaccharide consisting of glucose units of α (1-6) chains that are sequential to each other and is commercially available which can be applied in the food industry or biomedical fields. Dextran is synthesized by bacteria by utilizing sucrose. Dextran-producing bacteria can be isolated from fruits or foods that contain sugar in the form of sucrose. This study aims to obtain bacterial isolates that have the potential to produce dextran and can be utilized in the biomedical field, especially in the delivery of nanoparticle-based drugs. Dextran producing bacteria isolated from red matoa fruit, yellow matoa fruit and liberica coffee fruit. Matoa fruit is known to contain carbohydrates (sugar) of 21,100 mg/100 gr fruit and liberica coffee fruit has a sucrose content of 9,000 mg/100 gr fruit. Bacterial isolation was carried out using the spread plate method on selective medium with a concentration of 15% sucrose. The results showed that seven isolates had the characteristics of dextran-producing bacteria, two of them were isolated from red matoa fruit with the codes MTM1 and MTM3, three isolates from yellow matoa fruit with the codes MTK1, MTK2 and MTK4 and two isolates from liberika coffee beans with the codes KPL2 and KPL3. Of the seven isolates, three of them have the potential to produce dextran, namely isolates MTM3, MTK4 and KPL3, the highest production yield was isolate MTK4 with a total dry weight of 35.1 g/L and a dextran viscosity of 1.325 cP at concentration 2.5%. The resulting dextran can be used as a nano-based drug delivery agent but the dextran is made at a higher concentration.
Key findings: Dextran, Production, Spread Plate, Sucrose, Viscosity.
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