CD Skripsi

Bioassay Isolat Bakteri Ureolitik Riau Dan Kemampuan Isolat Campuran Dalam Menghasilkan Kalsit

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ABSTRACT
Ureolytic bacteria are a group of bacteria that can produce urease enzymes to hydrolyze urea. Ureolytic bacteria are able to produce calcite (CaCO3) through the precipitation process. The purpose of this study was to determine the toxicity of five isolates of SP. 32, SP. 34, SP. 48, SP. 83, and SP. 84 and the ability of the five isolates to produce calcite. Bacterial bioassay was carried out by adding a population inoculum 106 CFU/mL with various concentrations of 0%, 5%, 10%, and 15% into a container containing juvenile tilapia (Oreochromis niloticus) with observation times of 6, 12, 18, 24, 48, 72, and 96 hours. Compatibility test was carried out by streaking bacterial cultures on nutrient agar medium that each bacterial was in the middle of the media, and other bacterial cultures were streaked lengthwise from the middle. Calcite precipitation test was carried out by adding mixed inoculum with a total population 106 CFU/mL in NB-U/Ca medium (Nutrient broth) with an incubation time of 7 days at a speed of 150 rpm. The results of this study obtained the five isolates were still safe for the fish juveniles at 6, 12 and 18 hours at a concentration of 5%. Compatible bacterial isolates were SP. 32, SP. 34 and SP. 83. The highest calcite yield was obtained by using mixed isolates of SP. 32, SP. 34 and SP. 83 with a total of 907 mg/100 mL.
Keywords : bioassay, CaCO3, mortality, ureolytic

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1703113629

Penerbit

Pekanbartu : Universitas Riau - FMIPA - Biologi., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1703113629

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

puja

Versi lain/terkait

Lampiran Berkas

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