CD Skripsi

Isolasi Dan Seleksi Bakteri Asam Laktat Penghasil Eksopolisakarida Dari Nira Tebu (Saccharum Officinarum L.)

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ABSTRACT
Lactic acid bacteria (LAB) are bacteria that are safe for human consumption. Some types of LAB are able to produce secondary metabolites including exopolysaccharides (EPS). EPS-producing LAB has been isolated from various ingredients that contain high sugar sources so that it can be found in sugarcane sap (Saccharum officinarum L.). A fairly high sugar content is a suitable medium for the growth of EPS-producing LAB. This study aims to isolate EPS-producing LAB from sugarcane sap and analyze the EPS production capability of selected LAB isolates. The isolates of LAB were obtained using the method of dilution on MRSA medium (deMan Rogosa and Sharpe Agar) contained 0,5% CaCO3, then characterized based on macroscopic, microscopic, biochemical and physiological properties. The isolates of LAB were selected for their ability to produce EPS using MRSA media with the addition of 10% sucrose. LAB was further tested for its potential in producing EPS. The results of the study obtained LAB isolates in sugarcane sap, namely NT1, NT2, NT3, NT4, NT5, NT6, NT7, and NT8. The eight isolates obtained had the form of cocci and coccobacilli cells, gram positive, able to ferment carbohydrates, unable to produce indole, negative catalase and non-motile. The selection of EPS-producing LAB obtained 3 best isolates, namely NT3, NT4 and NT8. EPS produced by each isolate is 2.893 g/L, 2.890 g/L, and 3.090 g/L.
Key findings: Exopolysaccharides, lactic acid bacteria, Saccharum officinarum L., secondary metabolites, sugarcane sap.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1903156556

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Biologi., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1903156556

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

PUJA

Versi lain/terkait

Lampiran Berkas

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