CD Skripsi

Efek Suplementasi Vitamin D Terhadap Viabilitas Sel Punca Mesenkimal

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ABSTRACT

Mesenchymal stem cells (MSC) are multipotent progenitor cells with the ability to self-renew or divide into various types of cells. Mesenchymal stem cell culture is a method to increase the number of MSCs become sufficient to be used for therapy. One of the challenges in MSC culture is to maintain MSC viability due to the long-term culture process. This study aimed to determine the effect of vitamin D supplementation on MSC viability. The study design was a quasi experimental laboratory. Mesenchymal stem cells were isolated from Wharton's jelly umbilical cord using the explant method and then cultured with or without vitamin D supplementation for 24 and 48 hours. MSC viability was evaluated using the trypan blue exclusion test method. The difference in the mean viability of MSC in the experimental groups was tested using the One-Way Anova. To analyze the differences in the mean viability of MSC based on exposure time, an independent sample t test was carried out. The results showed that the mean of MSC viability was comparable following vitamin D supplementation of 50 and 100 nM for 24 hours (p=0.593) and 48 hours (p=0.517). In addition, there was no significant difference in MSC viability based on duration of exposure either 24 (p=0,228) or 48 hours (p=0,087). In conclusion, vitamin D supplementation in the culture medium did not affect the MSC viability.

Keywords : Mesenchymal stem cell, trypan blue exclusion test, viability, vitamin D

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2008111246

Penerbit

Pekanbaru : Universitas Riau - FK – Pendidikan Dokter., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2008111246

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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