CD Skripsi

Efek Suplementasi Vitamin D Terhadap Doubling Time Sel Punca Mesenkimal

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Mesenchymal stem cells (MSCs) are miltipotent progenitor cells that have the ability to self-renew and differentiate into mesodermal cell lines. Cell culture is a common produce to archieve an adequate nummber of MSC source. Many factors influence MSCs culture, such as culture condition and MSC source. In addition to its role bone metabolism, vitamin D is suggested to affect the rate of cell proliferation. This study aimed to determine the effect of vitamin D supplementation on MSC doubling time. This was a laboratory experimental study with a quasi- experimental design. Mesenchymal stem cells were isolated from Wharton's jelly using the explant method. Mesenchymal stem cells were divided into group of vitamin D 50 and 100 nM incubated for 24 hours and 48 hours while controls were treated with 0,01% ethanol absolute. The number of MSC doubling time was measured by the tryphan blue exclusion test method followed by the calculation of doubling time. Statistical analysis was done by the One-Way ANOVA test. Furthermore, Two-Way ANOVA was conducted to analyze the interaction of the concentration and duration of vitamin D supplementation on the doubling time of MSC. The results showed no significant difference between the dubling times of MSc treated vitamin D at ether a concentration at 50 and 100 nM compared with control group (p>0.05). However, there was a significant interaction between vitamin D concentration and exposure duration on MSC doubling time (p=0,0291). Vitamin D supplementation with concentration of 50 nM for 48 hours increased the doubling time of MSC compared to those supplemented for 24 hours. In conclusion, vitamin D supplementation may affect the doubling time of MSC.

Keywords: Vitamin D, doubling time, mesenchymal stem cells

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2008113767

Penerbit

Pekanbaru : Universitas Riau - FK – Pendidikan Dokter., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2008113767

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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