Image of Isolasi Dan Karakterisasi Lakase Trichoderma Asperellum Lbkurcc1 Serta Potensinya Dalam Bioremediasi Zat Warna Tekstil Metil Biru = Isolation And Characterization Of Trichoderma Asperellum Lbkurcc1 Laccase And Its Potential In The Bioremediation Of The Textile Dye Methyl Blue
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Isolasi Dan Karakterisasi Lakase Trichoderma Asperellum Lbkurcc1 Serta Potensinya Dalam Bioremediasi Zat Warna Tekstil Metil Biru = Isolation And Characterization Of Trichoderma Asperellum Lbkurcc1 Laccase And Its Potential In The Bioremediation Of The Textile Dye Methyl Blue

Background and objectives: Laccase is a multi-copper oxidase, a green catalyst in dye bioremediation. One of the laccase producers is Trichoderma asperellum LBKURCC1 and is utilized in the bioremediation of textile dye Methyl Blue (MB). MB is a triphenylmethane dye, and is widely used in the textile industry, is toxic and difficult to degrade. Methods: The potential of T. asperellum LBKURCC1 in degrading MB was tested using live culture and the enzyme it produced. Laccase isolation was carried out by 0-80% ammonium sulfate precipitation, 10 kDa ultrafiltration dialysis, and sephadex G-100 gel filtration chromatography, SDS-PAGE electrophoresis, and then characterized. Results: The biodegradation of MB was 85.19%, gel filtration column obtained laccase A and B, laccase A showed 2 bands, which represented 2 isozymes of laccase with BM 60 kDa and 66 kDa. Laccase A has a purity level of 12ᵡ, while laccase B is flow through. Both Laccase A and B had optimum pH values of 4.5 and 5.5, and optimum temperature of 45°C. The thermal stability of laccase A and B were ≤50°C and ≤60°C, respectively. The Km, Vm, and Kkat values of laccase A were 4570.526 µM, 263.158 µM/s, and 102.2x104 s-1, respectively. Laccase A showed increased activity in the presence of Cu2+ ions and urea, while other substances inhibited its activity. Laccase A also rendered MB non-toxic to Artemia salina shrimp larvae after degradation. Conclusion: Laccase is produced extracellularly and successfully enables biodegradation, causing the MB’s toxicity to change from moderate to non-toxic.

Keywords : Laccase, bioremediation, methyl blue, T. asperellum LBKURCC1 fungus, Artemia salina shrimp larvae

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Perpustakaan Universitas Riau 1610347937
1610347937
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1610347937
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Pekanbaru : Universitas Riau FMIPA S3 Ilmu Kimia.,
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Indonesia
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1610347937
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DISERTASI S3 ILMU KIMIA
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