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Evaluasi Sifat-Sifat Probiotik Untuk Lactobacillus Fermentum Inacc B1295 Dan Pediococcus Pentosaceus Strain 2397 Dengan Dan Tanpa Enkapsulasi Hidrogel Cellulose Microfiber Dari Daun Kelapa Sawit
Probiotics are defined as live microorganisms that when consumed in adequate amounts can provide health benefits to their host. This study aimed to evaluate the properties of cell surface hydrophobicity, autoaggregation and coaggregation of Lactobacillus fermentum InaCC B1295 and Pediococcus pentosaceus strain 2397 encapsulated with or without cellulose microfibre hydrogels from oil palm leaves. This study was carried out experimentally using a completely randomised design (CRD) with four treatments of Lactobacillus fermentum InaCC B1295 and Pediococcus pentosaceus strain 2397 cells encapsulated and not encapsulated with cellulose microfiber hydrogel from oil palm leaves, and each treatment was repeated four times, so that 16 experimental units were obtained. The parameters observed in this study were surface cell hydrophobicity, autoaggregation and coaggregation. The results showed that Lactobacillus fermentum InaCC B1295 and Pediococcus pentosaceus strain 2397 encapsulated with cellulose microfiber hydrogel from oil palm leaves significantly affected the values of hydrophobicity, autoaggregation, and coaggregation. Encapsulation with cellulose microfiber hydrogel caused both bacterial cells increased the cell surface hydrophobicity value by 10−18%, decreased autoaggregation ability of Pediococcus pentosaceus strain 2397 by 17%, while encapsulated cells of Lactobacillus fermentum InaCC B1295 increased by 10%. In comparison to pathogenic bacteria, Lactobacillus fermentum InaCC B1295 and Pediococcus pentosaceus strain 2397 exhibited a greater coaggregation value with other lactic acid bacteria.
Keywords: probiotics, Lactobacillus fermentum, Pediococcus pentosaceus, cellulose microfiber, oil palm leaves
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