CD Tesis

Optimasi Media Dan Waktu Fermentasi Untuk Produksi Metabolit Sekunder Dari Jamur Endofit Fusarium Oxysporum LBKURCC 41

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Fusarium oxysporum is one of endophytic microbes isolated from dahlia tuber (Dahlia variabilis) which has the ability to produce secondary metabolites. The purpose of this research was to find the optimum condition of fermentation to producing secondary metabolites. Corn, potato, and sweet potato and combination of them with particle size of 80 mesh were used as carbon sources to produce secondary metabolite for 5, 10, 15, 20, and 25 days respectively. Etyl acetate was used to extract secondary metabolites. The result of metabolites screening showed that secondary metabolites extracted was contained terpenoid group. The separation of the chemical constituents was performed using Thin Layer Chromatoghraphy and High Performance Liquid Chromatoghraphy (HPLC) depicted 4 components. Antimicrobial activity was examined against Candida albican, Escherichia coli and Staphylacoccus aureus. A serial of metabolite concentrations 5.7 mg/ml, 3.8 mg/ml, and 1.9 mg/ml were used. The optimum inhibition against microbial pathogen growth was showed by corn for 15 day fementation (C15). The inhibition zone against Candida albicans, E.coli, and S. aureus were 7.42±0.29, 12.85±1,43 and 13,88±1,51 mm respectively at 5.7 mg/ml metabolite concentration.
Keywords: Antimicrobial, Fusarium oxysporum, optimization, secondary metabolite, terpenoid.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 05. 219 (0006)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia., 2019

Deskripsi Fisik

xii, 118 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 05. 219 (0006)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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