Image of Reidentifikasi Fungi Endofit Lbkurcc40 Dari Umbi Tanaman Dahlia (Dahlia Variabilis) Menggunakan Sekuens Its Rdna
Penanda Bagikan

CD Tesis

Reidentifikasi Fungi Endofit Lbkurcc40 Dari Umbi Tanaman Dahlia (Dahlia Variabilis) Menggunakan Sekuens Its Rdna

LBKURCC40 is endophytic fungi which was isolated from orange flowering dahlia tubers of plants from outside Padang, West Sumatra. Identification by Lorenita (2013), Hendris (2015) and preliminary studies showed conflicting results, indicating that the isolate was not pure. Therefore purification and reidentification of LBKURCC40 was done to establish its correct identity. This study conducted serial purification to obtain pure isolates and molecular reidentification to ensure the species and phylogenetic relationship. Purification was done by serial dilution method from 10-1-10-30, and identification of microscopic morphology by using a light microscope Olympus CX41. Chromosomal DNA was isolated from three days old fungal mycelia using Wizard Genomic Purification kit (Promega Co Madison, USA), and amplification by PCR was performed using primers for RPB2, Tef1 and rDNA ITS. The results of the purification produced two sepparate isolates, namely LBKURCC40A and LBKURCC40B, and identification of macroscopic and microscopic morphology showed LBKURCC40A and LBKURCC40B to be Aspergillus sp. Chromosomal DNA fungi LBKURCC40B and LBKURCC40A each had a molecular weight of 11.562 and 13.303 pb. PCR amplification using primers for RPB2 and Tef1 was not successful, but the ITS rDNA at the annealing temperature of 45oC produced PCR products of 573 bp and 624 bp. The results of phylogenetic analysis using ITS-1 and ITS-2 rDNA sequences showed the species of LBKURCC40A was A. fumigatus and LBKURCC40B was A. flavus with levels of the similarity of 100% for each strain. Phylogenetic analysis showed that LBKURCC40A indeed formed a separate cluster together with A. fumigatus IFM 54 307, and is separated from the LBKURCC40B cluster that forms a separate cluster with A. flavus KAM8.

Key words: A. flavus, A. fumigatus, Internal Trascribe Spacer, rDNA

Ketersediaan
#
Perpustakaan Universitas Riau 10 05. 217 (0008)
10 05. 217 (0008)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
10 05. 217 (0008)
Penerbit
Pekanbaru : Universitas Riau – PASCA SARJANA – KIMIA.,
Deskripsi Fisik
xv, 68 hlm.;ill.; 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
10 05. 217 (0008)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
PASCASARJANA (MAGISTER) KIMIA
Info Detail Spesifik
-
Pernyataan Tanggungjawab
Versi lain/terkait

Tidak tersedia versi lain

Lampiran Berkas
Komentar
Anda harus masuk sebelum memberikan komentar