CD Tesis

Uji Aktivitas Enzim Laccase Produksi Trichoderma Asperellum Lbkurcc1

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Almost all the dyes used in the textile and textile products industry is a synthetic dye. The is most often used are azo dyes. The liquid waste dye generated will cause environmental pollution and are likely to be carcinogenic. Liquid waste dye can be degraded by using microorganisms such Trichoderma asperellum LBKURCC1 which capable to degrade azo direct violet 51 and CIRB5 anthraquinone dyes. This fungi produce extracellular laccase enzyme that plays a role in the process of oxidation and degradation of anthraquinone and azo dyes. Laccase enzyme is catalyzes the oxidation of various types of inorganic and organic compounds such as phenolic compounds. Objectives of this study was to determine the ability of T. asperellum LBKURCC1 to produce laccase enzyme in two media containing two different carbon sources (rice husks and Avicel), determines the optimal incubation time, and comparing the activity of the enzyme laccase in media production with commercial laccase enzyme activity (laccase enzymes from Rhus vernicifera). Laccase enzyme activity assay was carried out using guaiacol as substrate, incubated for 24h at 40 °C, pH 5,5 and measured using a spectrophotometer at a wavelength of 490 nm. The results showed that the fungus T. asperellum LBKURCC1 is capable to produce extracellular laccase enzyme activity of 0,0956 ± 0,0037 U/L (rice husk), 0,0952 ± 0,0010 U/L (Avicel), while commercial enzyme activity was 0,0247 ± 0,0002 U/L. Optimal incubation time for the laccase enzyme production is 48 hours (in both media). Statistical analysis showed significant differences (p

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 05. 217 (0009)

Penerbit

Pekanbaru : Universitas Riau – Pasca Sarjana – Kimia., 2017

Deskripsi Fisik

xv, 59 hlm.;ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 05. 217 (0009)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FIKRATUL

Versi lain/terkait

Lampiran Berkas

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