CD Tesis

Optimalisasi Konsentrasi Surfaktan, Nitrogen, & Karbon Media Produksi Lakase T. Asperellum LBKURCC 1 Secara Fermentasi Padat Menggunakan Batang Padi Dan Serbuk Gergaji Sebagai Substrat

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Laccase (benzenediol: oxygen oxidoreductase, EC 1.10.3,2) is an important industrial enzyme used in the paper, food and textile industries. It is produced by many different organisms, including filamentous fungi. Trichoderma asperellum LBKURCC1 is a strain isolated from Riau soil, which can produce laccase by solid state fermentation (SSF) of rice husk and rice straw. The aim of this work was to optimize SSF production of laccase from rice straw, through optimizing nitrogen, carbon and surfactant supplements to the fermentation media. Laccase enzyme activity assay was carried out using ABTS as a substrate, incubated for 5 min at 40 °C, pH 5,5 and measured using a spectrophotometer at a wavelength of 436 nm. Effect of surfactant, nitrogen supplement, and carbon supplement were evaluated by using a Central Composite Design (CCD) and surface response analysis. The concentration of the surfactant, Tween-20, at all concentration levels tested had no significant effect to the model of rice straw and sawdust as a substrate. In contrast, the nitrogen supplement and carbon supplement concentration were variables that could significantly enhance laccase activity to rice straw and sawdust as a substrate (P

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 05. 220 (0001)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia., 2020

Deskripsi Fisik

xv, 99 hlm.; ill.; 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 05. 220 (0001)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PASCASARJANA (MAGISTER) KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

Lampiran Berkas

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