ABSTRACT Actinomycetes is a microorganism that can produce secondary metabolites such as antibiotics. Secondary metabolites can use to control microbial pathogen such as Staphylococcus aureus, Salmonella typhimurium, Shigella flexneri, Pseudomonas aeruginosaandEscherichia coli. M1.T1, M1.T3, M1.T4 dan M1.T5 actinomycetes isolates had been isolated from Vegetable Garden Soil of Pekanbaru c…