CD Skripsi
Uji Aktivitas Antibakteri Senyawa Makrosporin Dari Ekstrak Fungi Endofit Botryosphaeria Rhodina
Endophytic fungi have the ability to synthesize a secondary metabolite compounds
with the potential for large-scale antimicrobial development in a short period of
time while causing no environmental damage. The goal of this study was to look at
the life cycle of the endophytic fungus Botryosphaeria rhodina and to evaluate the
antibacterial activity of extracts and macrosporin compounds produced by the
fungus. Secondary metabolites were isolated from an n-hexane extract of the
endophytic fungus B. rhodina using Vacuum liquid chromatography. Analysis of
the life phase of the endophytic fungus B. rhodina using a dry weight growth curve
revealed that the log phase occurred on days 1 to 2, the stationary phase occurred
on days 3 to 9, and the death phase occurred on days 10. The compound
characterization yielded macrosporin compounds in the form of orange colored
crystals weighing up to 157 mg from FIV and FV, which were identified using
spectroscopy techniques. The results of the antibacterial test carried out by the agar
diffusion method on the n-hexane and methanol extracts at concentration
500 μg/mL, n-hexane fractions at concentration 300 μg/mL, and macrosporin
compounds at concentration 100 μg/mL, showed moderate antibacterial activity
with a zone diameter of inhibition ranged from 10-20 mm against Gram-positive
bacteria Staphylococcus aureus, Bacillus subtillis, Bacillus cereus, and Gramnegative
bacteria Salmonella typhimurium, Vibrio algynolyticus, and Vibrio
parahaemolyticus
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