CD Tesis
Pemanfaatan Air Lindi Sampah Perkotaan Secara Alami Dengan Dosis Berbeda Terhadap Kelimpahan Dan Komposisi Kimia Chlorella Sp
During the decomposition process of domestic waste, leachate was
produced. This waste water is rich in organic matters such as nitrate, phosphate,
ammonia, Fe and Mn and it may be used as nutrient resources for plants,
including microalgae. One of microalgae that commonly cultured is Chlorella
sp. In the culture of this algae, the nutrient source for the algae may be originated
from liquid fertilizer, palm oil liquid waste and other sources. However, the
domestic waste leachate was never been used as nutrient source of this algae. The
purposes of this study are to understand the effectiveness of the domestic waste
leachate for growing Chlorella sp in the laboratory scale; to understand the best
dosage of domestic leachate for culturing Chlorella sp; to understand the
relationship between leachate dosages with abundance and chemical composition
of Chlorella sp and also to understand the effectiveness of Chlorella sp that was
used as feed for Helostoma temmincki larvae as it is reflected in the survival and
growth of the larvae. There was CRD applied in this research. There were 5
treatments applied, namely 5% leachate (P1); 10% leachate ( P2); 15% leachate
(P3), 20% leachate (P4) and 25% leachate (P5). The leachate used in this study
was taken from the Muara Fajar Dumping Area. The liquid waste was directly
mixed with well water and then was used for Chlorella culture media. Chlorella
sp used in this study originated from the Microalgae Laboratory of Fishery and
Marine Science University, Pekanbaru. Parameters measured were growth and
abundance of Chlorella sp, specific growth rate, total biomass of Chlorella sp ;
proximate and amino acid content. The water quality parameters measured were
temperature, pH, DO, NH3, as well as COD and BOD. The fish larvae used in this
study was the 3 days old H. temmincki larvae (around 0.0062 gr) and they were
reared for 28 days. The highest abundance of Chlorella sp. was obtained in P2, it
was 5,516,000 cells/ml, while for the lowest population was in P5 (2,950,000
cells/ml). In P2, the peak of Chlorella growth was in 12th day, while that of the
P5 was on the 8th day. The leachate dosages, however, do not affects the chemical
composition of the Chlorella in general, but algae in P2 shown the highest content
of protein (32.82 %), while the lowest was in P5 (30.90%). The amino acid of the
algae in all treatment do not shown any differences. The survival and growth of
fish larvae fed with Chlorella sp was better than that of the larvae with no algae
treatment. The fish larvae fed with algae grew better. After being fed with algae
for 28 days, the body weight of the larvae was 0.0081 gram/ larva, while that of
the larvae with no algae was 0.0034 gram/ larva. Data obtained in this study
indicate that dose of leachate significantly affects the abundance of Chlorella sp.
The best dose was 10% leachate and in the peak (12th day) the algae population
was 5,516,000 cells/ml. the leachate dose, however, do not affect the chemical
composition of the algae. Data on algae population indicate that the domestic
leachate water may potential as a nutrient source for Chlorella sp culture. The
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Chlorella cultured in the leachate enriched media was potential as larvae feed as
it improved the growth of H. temmincki larvae. To get more information on the
potential of domestic leachate for culturing algae, it is necessary to culture
different types of algae using the leachate enriched media and to provide leachate
media cultured algae to many fish larvae species.
Key Words : Chlorella sp, Culture Media, Water Leachate, Growth
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