CD Tesis

Pemanfaatan Enzim Alcalase Pada Pembuatan Hidrolisat Protein Ikan Malong (Congresox Talabon) Sebagai Inhibitor Enzim Α-Glukosidase

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Malong fish (Congresox talabon) is a type of demersal fish that has
important economic value and makes a large contribution to the number of fish
catches in Bengkalis Regency. Malong fish caught by fishermen are usually not in
intact condition, because the swim bladder (lupe) has been taken to be sold dry at
a fairly high price on the market, so usually only the swimming bubble is used, so
that the malong fish meat is not utilized. Malong fish meat has a fairly high
nutritional value, especially protein content. The relatively high protein content in
the fish meat can be used as a raw material in the manufacture of fish protein
hydrolyzate. The content of the amino acids leucine, arginine, lysine, alanine,
phenylalanine, isoleucine and methionine in the protein hydrolyzate of malong
fish is able to control blood glucose levels by inhibiting the α-glucosidase enzyme.
The purpose of this study was to obtain the proportions of the body parts
of the mackerel and look at the chemical composition of the fish meat and flour,
look at the characteristics and yield of the malong fish meal, obtain the best
concentration of alcalase enzymes in the protein hydrolysate of mackerel based
on the value of antioxidant activity, degree of hydrolysis, and yield, as well as to
determine the characteristics of amino acids and the hydrolyzate inhibition of
protein malong fish against α-glucosidase enzymes.
This research was carried out in May 2022 at the Fisheries Products
Technology Laboratory, Fisheries Products Chemistry Laboratory, Faculty of
Fisheries and Maritime Affairs, University of Riau. Amino acid analysis at the
Nawa Agna Laboratory in Bogor, α-glucosidase analysis at the Laboratory and
Center for Biopharmaca Studies IPB, and for lyophilization using a freeze dryer
at the Pekanbaru Center for Product Development and Industrial
Standardization. The main ingredient in the form of malong fish is obtained from
the catch of fishermen from Bengkalis Regency, Riau. Materials and tools used
are all supporting chemical materials and tools as well as other supporting
materials.
This study used an experimental method and the implementation was
carried out in two stages which included the preparation stage of the malong fish
and the manufacture of flour to produce the malong fish meat flour and then the
proximate analysis of the meat and flour was carried out, followed by the
calculation of the yield of the malong fish meal. The second stage was the
manufacture of protein hydrolyzate of malong fish using different alcalase enzyme
concentrations. The protein hydrolyzate obtained was calculated by yield, degree
of hydrolysis, and antioxidants to produce protein hydrolyzate with the best
alcalce enzyme concentration and then analyzed for amino acids and α-
glucosidase enzyme inhibitors. The data obtained were analyzed statistically with
analysis of variance (anava) and then tabulated into tables and graphs. Based on
the analysis of variance, if F-count F-table at 99% confidence level then H0 is rejected, then
BNJ and BNT follow-up tests are carried out.
The chemical composition of the malong fish meat obtained was water :
ash : protein : fat sequentially 72.98 : 3.47 : 60.05 : 0.12%. While the chemical
composition of malong fish meal is 8.16 : 4.62 : 64.78 : 1.76%. The
characteristics of the fish meal produced are brownish yellow in color, has a
smooth texture and has a distinctive aroma of dried fish and slightly smells of oil.
The yield of fish meal produced was 93.51%. The use of alcalce enzymes with a
concentration of 2.5% (w/v); temperature 55oC; pH 7.5; and hydrolysis time for 2
hours is the best treatment which has a yield value of 59.04%, the optimum degree
of hydrolysis value is 77.45% with an IC50 value

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2010241986

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Kelautan., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2010241986

Tipe Isi

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Tipe Media

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Tipe Pembawa

-

Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

Lampiran Berkas

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