CD Skripsi
Produksi Biomassa Dan Enkapsulasi Protein Sel Tunggal (Pst) Dari Bakteri Konsorsium Bacillus Cereus
Abstract
Commercial feed is relatively expensive, therefore there must be a solution for artificial feed that is relatively cheap, efficient and high in nutrition. Probiotic bacteria can kill pathogenic bacteria so that they can be an alternative to fish feed. The Bacillus cereus consortium bacteria are probiotic bacteria that have the ability to inhibit the growth of pathogenic bacteria and have the potential to be developed as a single cell protein agent for additional fish feed. The aims of the research were to produce biomass produced by the B. cereus consortium bacteria, carry out encapsulation to protect the B. cereus consortium bacterial biomass from conditions that could damage bacterial cells, and to test the effectiveness and viability of the B. cereus consortium bacterial encapsulation. The research was conducted from September to December 2022. The method used in the research was an experimental method in which the B. cereus consortium bacteria produced their biomass in modified growth media of sago liquid waste and egg whites, then the bacterial cells were harvested by centrifugation. After that the bacterial cells were encapsulated, the microcapsules formed were then tested for effectiveness and viability for 75 minutes. Based on the research results, the production of bacterial biomass in one harvest was 2.1713 grams in wet weight and 1.6474 grams in dry weight. Bacterial cells are effectively encapsulated with alginate and chitosan ditambah acetic acid coating. In the effectiveness test on mesophilie and psychrophilic temperatures, neutral and alkaline pH, as well as physiological NaCl solution were quite effective in maintaining its effectiveness. Meanwhile, at thermophilic temperature and acidic pH, it undergoes shape changes such as lysis in microcapsules. In the viability test using the TPC method and Gram staining the bacterial cells experienced colonial growth and the bacterial cells after being encapsulated were still alive.
Keywords: Single Cell Protein, Bacillus cereus, Encapsulation, effectiveness and viability test
1) Student of the Faculty of Fisheries and Marine, University of Riau
2) Lecturer at the Faculty of Fisheries and Marine, University of Riau
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