CD Tesis

Eksplorasi Dan Karakterisasi Bakteri Penambat N Non Simbiotik Dan Bakteri Nitrifikasi Pada Tanah Gambut Yang Diaplikasi Abu Boiler

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Peatlands in Indonesia are widely used for the development of plantation crops, one of which is oil palm. The development of peatlands faces many obstacles, such as the reaction of acidic to very acidic peat soils with high cation exchange capacity (CEC) and the availability of nutrients in a bound form. These conditions do not support plant growth in general.
The solution to solve the problems in peatlands is by applying fertilizers and ameliorants. However, this activity is less efficient and takes up the largest portion of costs, namely 30% of the total production costs. We need another alternative to support the more efficient provision of nutrients, one of the solutions is the use of bacteria that can provide nutrients for plants such as non-symbiotic nitrogen-fixing bacteria.
Non-symbiotic N-fixing bacteria are one of the biological potentials in plant cultivation areas. This bacterium is used as a fixing agent for free N in the air as well as a soil aggregate stabilizer. One example is Azotobacter and Azosprilum. Seeing the condition of acidic peatlands, non-symbiotic N-fixing bacteria are less developed. Improvement of soil chemical properties can provide optimal conditions for the growth of non-symbiotic N-fixing bacteria, one of which is by administering ameliorants in the form of boiler ash. The presence of non-symbiotic N-fixing bacteria can also be used as information for the presence of other bacteria such as nitrifying bacteria
The purpose of this experiment is to isolate and characterize non-symbiotic N-fixing bacteria isolates and nitrifying bacteria from peat soils that are applied to boiler ash. Sampling locations were taken at the Minamas Research Center, Riau Province, and the Soil Laboratory, Faculty of Agriculture, University of Riau. Determining the location of sampling by purposive sampling.
The research was carried out in several stages, namely the stage of adding boiler ash to peat soil, analyzing soil pH, taking soil samples, isolating bacteria, purifying bacteria, and observing selected bacterial isolates. The study was carried out using a completely randomized design consisting of three treatments and three replications to obtain 9 experimental units. The treatments in this study included A: Peat soil without the application of boiler ash, B: Soil with the application of 1.5 tons/ha/year of boiler ash, and C: Soil with the application of 3 tons/ha/year of boiler ash. The criteria used as observation parameters in the identification of these bacterial isolates were the morphological characteristics of the bacteria including the macroscopic and microscopic characteristics of the bacterial isolates, biochemical tests, and physiological tests. Data on the number of bacteria obtained were analyzed statistically using variance, then followed by the Duncan Mean Range Test (DMRT) at 5% level.
A total of 36 isolates of non-symbiotic N-fixing bacteria were successfully isolated on Ashby's and NFb medium. Also obtained 36 Nitrifying bacteria isolates from TSIA and Burk's medium. The test results for the potential of non-
symbiotic N-fixing bacteria showed that 18 isolates were able to form a pellicle on Ashby's medium and 18 isolates were able to change the color of the medium on NFb medium. The potential test to produce ammonium and nitrate was measured using a spectrophotometer. Isolates A32 and B32 are isolates that have a low ability to produce ammonium and nitrate so they can be used on peat soils. The results of Gram staining of isolates of non-symbiotic N-fixing bacteria and nitrifying bacteria were all Gram-negative. The results of the catalase test showed that all isolates of non-symbiotic N-fixing bacteria and nitrifying bacteria were classified as aerobic bacteria.
Keywords: peat soil, boiler ash, symbiotic N fixing bacteria, nitrifying bacteria

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2010241682

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Pertanian., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2010241682

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

Lampiran Berkas

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