CD Skripsi

Isolasi Bakteri Escherichia Coli Dari Tambak Udang Vaname (Litopenaeus Vannamei) Dan Sensitivitasnya Terhadap Antibiotik Dalam Bidang Ilmu Kelautan

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ABSTRACT
Vannamei shrimp (Litopenaeus vannamei) is a fishery commodity that is widely cultivated in various parts of Indonesia. The main problem that is often found in vannamei shrimp production failure is poor water quality during the rearing period. This study aims to isolate and identify E.coli contained in water, sediment, and meat of vannamei shrimp cultivated in shrimp ponds and examined the sensitivity against antibiotic. The research was conducted from March to May 2023, the Technical Implementation Unit (TIU) Vannamei Shrimp Ponds of the Maritime Affairs and Fisheries Service, Tanjung Punak Village, Rupat Utara District, Bengkalis Regency, Riau. The methods used in this study were the survey method. The Most Probable Number (MPN) method was employed for E.coli isolation to calculate bacterial density, and the Kirby-Bauer method was used to test bacterial sensitivity to antibiotics. Based on the identification of the 6 isolates carried out, it was found that only 2 isolates were positively identified as E.coli bacteria, namely SD C 3.1 and IP B 1.1 which were isolates from sediment and Wastewater Treatment Plant (WWTP) pond. The diameter of the inhibition zone for sediment isolate RF01 against the antibiotic chloramphenicol was 18.75 mm, indicating intermediate sensitivity. On the other hand, WWTP isolate RF02 exhibited an inhibition zone of 17 mm for chloramphenicol, indicating intermediate sensitivity as well. However, it showed resistance to penicillin with a diameter of 6.5 mm and resistance to ampicillin with a diameter of 2 mm.
Keywords: Antibiotics, Escherichia coli, Kirby-Bauer, MPN, Sensitivity, Vannamei Shrimp Pond
1) Student of the Faculty of Fisheries and Marine, University of Riau
2) Lecturer at the Faculty of Fisheries and Marine, University of Riau

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1904111775

Penerbit

Pekanbaru : Universitas Riau – Fakultas Perikanan Dan Ilmu Kelautan – Ilmu Kelautan., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1904111775

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU KELAUTAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

DAUS

Versi lain/terkait

Lampiran Berkas

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