CD Tesis
Karakteristik Hidrolisat Teripang Berunok (Paracaudina australis) dan Asam Amino Total Menggunakan Enzim Pepsin
This study was conducted to determine the characteristics of berunok sea cucumber hydrolysate (Paracaudina australis) and the type and level of total amino acids contained. This research was conducted using experimental methods, with a Non-Factor Complete Randomized Design consisting of 3 levels of enzyme concentration treatment (1%, 2% and 3%). In this study, the parameters tested were the proximate content of flour and hydrolysate of berunok as well as the type and content of total amino acids. The results showed that the chemical composition of berunok flour was water 11.78% (dw), ash 9.44% (dw), protein 61.67% (dw), fat 5.39% (dw) and carbohydrate by difference 11.72% (dw). The best treatment for the preparation of berunok protein hydrolysate using pepsin enzyme is 2% pepsin enzyme concentration. The proximate of berunok protein hydrolysate is water 6.84% (dw), ash 8.03% (dw), protein 74.72% (dw), and fat 2.79% (dw). The results of identification of types and levels of total amino acids found 17 types of amino acids with a total of 16.524% in hydrolysate berunok.
Keywords: Berunok, pepsin, protein hydrolysate and total-amino acids.
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