CD Skripsi
Uji Potensi Antimikroba Bakteri Asam Laktat (Bal) Cangkuak Semaung ( Pangium Edule Reinw) Terhadap Mikroba Patogen
Antimicrobials are substances that can inhibit or kill the growth of pathogenic microbes. Pathogenic microorganisms are microorganisms that can cause disease in other organisms. Antimicrobials include antibacterial and antifungal. Antibacterials are substances that can inhibit the growth of bacteria and can kill pathogenic bacteria, while antifungals are substances that can inhibit and kill pathogenic fungi. Lactic Acid Bacteria (BAL) are included in the group of good bacteria that usually have GRAS (Generally Recognized as Safe) status, which means they are safe for human consumption and potential as probiotics. BAL produces products in the form of lactic acid, and antimicrobial compounds in the form of bacteriocin. This bacteriocin was considered capable of being used as a natural antibiotic, because it was able to inhibit the growth of pathogenic bacteria. This research was conducted to test the antimicrobial activity of the Cangkuak Semaung isolate of the Lactic Acid Bacteria (LAB) strain against the growth of pathogenic microbes. The methods used in this research were disk diffusion for the antibacterial test and two-layer plate and spread plate methods for the antifungal test. The results of the antibacterial test showed that the inhibition zone for Escherichia coli (5-9 mm) was larger than for Staphylococcus aereus (3-4 mm) and the inhibition zone for Acinetobacter baumannii and Klebsiella pneumoniae was smaller, namely (0.0-0.9 mm). and (0.0-0.4 mm). The results of the first screening for the antifungal test showed that there were 38 LAB isolates out of 45 LAB isolates that were able to degrade AFB1. Then, during repeated screening of selected isolates, there was 1 isolate that was thought to be able to inhibit the growth of Aspergillus flavus.
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