CD Skripsi

Isolasi Senyawa Metabolit Sekunder Ekstrak Etanol Daun Benger (Lagerstroemia Ovalifolia Teijsm. & Binn) Sebagai Aktivitas Antioksidan Dan Inhibitor Enzim Tirosinase

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Skin is an organ that covers the entire outer surface of the human body and plays an important role as a protector. The skin can experience damage caused by free radicals from ultra violet (UV) rays, namely skin cancer and hyperpigmentation. Therefore, it is necessary to carry out studies to find alternative compounds from natural ingredients that have the potential to act as antioxidants and inhibitors of the tyrosinase enzyme. The benger plant (Lagerstroemia ovalifolia Teijsm. & Binn) from the Lythraceae family has been used in herbal medicine and has anti- inflammatory properties. This study aims to isolate secondary metabolites from the ethanol extract of L. ovalifolia leaves and test antioxidant activity using the DPPH method and tyrosinase enzyme inhibitor activity, both of which are guided qualitatively using the TLC bioautography method, while quantitatively using the microplate reader (ELISA) method. Isolation started from ethanol extraction of L. ovalifolia leaves using 96% ethanol solvent and phytochemical test was carried out to determine secondary metabolite groups. Obtained IC50 of antioxidant DB extract was 52,960 and IC50 antityrosinase was 4.364,424. DB was fractionated using a diaion HP20 stationary phase column with a mobile phase of water and methanol to obtain 4 fractions and then subjected to a TLC bioautography test for antioxidants and tyrosinase inhibitors. The DBD4 fraction is an active fraction for antioxidants with an IC50 of 39,503 and an anti-tyrosinase IC50 of 1.666,493. DBD4 was separated using vacuum liquid chromatography (VLC) with a stationary phase of silica gel GF254 and a mobile phase of dichloromethane, ethyl acetate and methanol to obtain 8 subfractions and then subjected to TLC bioautography antioxidant and tyrosinase inhibitor tests. The DBD4V4 subfraction is a subfraction that is active in antioxidants with an IC50 of 65,536 and an antityrosinase IC50 of 1.908,165. DBD4V4 was purified using semi-preparative high performance liquid chromatography (HPLC) to obtain 6 isolates with dominant peaks. Of the 6 isolates, TLC bioautography tests were carried out for antioxidants and tyrosinase enzyme inhibitors, resulting in 4 active isolates. The compound purity test was carried out using analytical HPLC, resulting in 2 active isolates which had 1 dominant peak at a wavelength of 254 nm with a retention time for isolate I14-DBD4V4 at 12,48 minutes and isolate I11-DBD4V4 at 18,61 minutes. Compounds with codes I14- DBD4V4 and I11-DBD4V4 were characterized to determine the structure of the compound using a Photodiode Array Detector (PDA), Fourier Transform Infrared (FTIR), and Ultra Performance High Chromatography (UPLC). Compound I14- DBD4V4 is a flavonoid compound, has a BM (molecular weight) of 432 g/mol, it is estimated that it is an apigenin-7-O-glycoside compound. Compound I11- DBD4V4 is a flavonoid compound, known to have 2 peaks with a BM of 448 g/mol which is thought to be a luteolin-7-O-glycoside compound and a BM of 302 g/mol which is thought to be a quercetin compound.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003126838

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003126838

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

mardiah

Versi lain/terkait

Lampiran Berkas

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