CD Skripsi

Produksi Pigmen Penicillium Sp. Lbkurcc27 Padamedia Cair Dengan Variasi Konsentrasi Sumber Nitrogen Berupa Urea

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Penicillium sp. LBKURCC27 is an isolate that can be used to produce orange pigment. The resulting color pigments can color silk and cotton fabrics well. This research aims to explore the potential of local isolates of Penicillium sp. LBKURCC27 as a source of natural orange pigment with variations in urea concentration as a nitrogen source for pigment production and cell growth. The research was carried out by producing pigments in PDB liquid media to which urea was added with varying concentrations of 1.5%; 2%; 2.5% and 3%, and without addition of urea. Pigment production is carried out at room temperature, in a dark environment and static conditions. The pigment produced was extracted with ethyl acetate and continued by evaporating the solvent until dry using a rotary evaporator. Based on visual observation, on the surface of the production media where urea was not added there were green mycelial and black spores, but on the production media with the addition of urea only white mycelial threads were visible (at 2.5% and 3%) and even almost no mycelia visible (1.5% and 2%). Based on the viability test, it was concluded that there were still viable mushrooms after fermentation for more than 40 days. The highest pigment production was at a urea concentration of 2% and the lowest at 0%. Increasing the urea concentration higher than 2% did not further increase pigment production. The cell mass of the Penicillium LBKURC27 fungus was lower with the addition of urea compared to media without urea because urea is toxic which inhibits fungal growth. The spectrum of pigments produced is dominated by red to orange colors.

Penicillium sp. LBKURCC27 is an isolate that can be used to produce orange pigment. The resulting color pigments can color silk and cotton fabrics well. This research aims to explore the potential of local isolates of Penicillium sp. LBKURCC27 as a source of natural orange pigment with variations in urea concentration as a nitrogen source for pigment production and cell growth. The research was carried out by producing pigments in PDB liquid media to which urea was added with varying concentrations of 1.5%; 2%; 2.5% and 3%, and without addition of urea. Pigment production is carried out at room temperature, in a dark environment and static conditions. The pigment produced was extracted with ethyl acetate and continued by evaporating the solvent until dry using a rotary evaporator. Based on visual observation, on the surface of the production media where urea was not added there were green mycelial and black spores, but on the production media with the addition of urea only white mycelial threads were visible (at 2.5% and 3%) and even almost no mycelia visible (1.5% and 2%). Based on the viability test, it was concluded that there were still viable mushrooms after fermentation for more than 40 days. The highest pigment production was at a urea concentration of 2% and the lowest at 0%. Increasing the urea concentration higher than 2% did not further increase pigment production. The cell mass of the Penicillium LBKURC27 fungus was lower with the addition of urea compared to media without urea because urea is toxic which inhibits fungal growth. The spectrum of pigments produced is dominated by red to orange colors.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003113748

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003113748

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

mardiah

Versi lain/terkait

Lampiran Berkas

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