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Isolasi Dan Uji Toksisitas Ekstrak Etil Asetat Dari Benalu Jambu Air (Dendrophthoe Pentandra (L.) Miq)
Dendrophthoe pentandra (L.) Miq is a plant from the Lorantaceae family that grows as a parasite on the guava plant, so it is called the water apple mistletoe. This research aims to isolate secondary metabolites and test toxicity in water mistletoe leaves (D. pentandra (L.) Miq) from ethyl acetate extract using the Brine Shrimp Lethality Test (BSLT) method. Secondary metabolite compounds were isolated from ethyl acetate extract using column chromatography, resulting in 4 fractions. Fraction 4 (F4) produces a pure compound in the form of a yellow powder with a melting point of 182-184°C, after which it is coded DPEA-F4. Characterization of the DPEA-F4 compound was carried out using a UV-Vis and FTIR spectrophotometer. Based on the spectrum of UV-Vis measurement results, the maximum absorption is at wavelengths of 255 and 354 nm, while the FTIR spectrum results show that there are absorption bands at 3167 and 3281 cm-1 which are O-H groups, at 2930 cm-1 indicate aliphatic CH groups, at 1658 cm-1 indicates a C=O bond group, while at 1602 cm-1 there is a C=C bond group (alkene) and at a wave number of 1201 cm-1 indicates the presence of a C-O bond group. Based on the results of characterization and phytochemical tests, the DPEA-F4 compound is a phenolic compound in the flavonoid group. Toxicity test analysis with BSLT produces an LC50 value of >1000 ppm, indicating that the ethyl acetate extract is non-toxic.
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