CD Skripsi

Isolasi Metabolit Sekunder Dari Kloroform Tumbuhan Paku Sarang Burung Fakultas Matematika Dan Ilmu Pengetahuan Alam Dan Uji Antibakteri Senyawa Fraksi 2 Ekstrak (Asplenium Nidus L.)

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Asplenium nidus Linn is a fern that belongs to the Aspleniaceae family and lives as an epiphyte. Other species from the Aspleniaceae family, such as A. robustum, A. caudatum, and A. tenerum, are known to contain secondary metabolite compounds, incluiding flavonoids, alkaloids, and terpenoids, which exhibit antibacterial, anticancer, and antioxidant activities. The limited information about the potential of this plant means that research into the antibacterial activity of A. nidus is still scarce. This study aims to isolate and characterize secondary metabolite compounds obtained from the compounds, fractions, and chloroform extracts of A. nidus and to determine their antibacterial activity. The sample was extracted using the maceration method with methanol as the solvent, and then fractionated using the liquid-liquid exctraction method with ethyl acetate, chloroform, and n-hexane as solvents. The chloroform extract was further separated using Vacuum Liquid Chromatography (VLC), producing six main fractions. From fraction II, a pure compound was obtained in the form of a yellowish-brown powder and was assigned the code 23-12-B-FII. The compound’s purity was tested using Thin Layer Chromatography (TLC), the Fisher Johns melting point test, and High-Performance Liquid Chromatography (HPLC) analysis. Compound 23-12-B-FII underwent characterization analysis and was elucidated using UV-Vis, FTIR, and NMR spectroscopy. The characterization results indicated that the compound obtained were of the phenolic group. The compound was tested for antibacterial activity using the microdilution method, and the Minimum Inhibitory Concentration (MIC) test results were 25 µg/mL for the compound, 125 µg/mL for the fractions, and 250 µg/mL for the extracts, while the Minimum Bactericidal Concentration (MBC) test results were 50 µg/mL for the compound, over 500 µg/mL for the fractions, and over 1000 µg/mL for the extracts. Data from the antibacterial activity tests indicate that the fern A. nidus has significant potential in inhibiting and killing the four pathogenic bacteria tested: S. typhimurium, V. alginolyticus, S. aureus, and B. subtilis.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2003113751

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2003113751

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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