CD Skripsi
Isolasi Dan Skrining Antibakteri Jamur Endofit Dari Kulit Batang Dan Daun Tumbuhan Leban (Vitex Pubescens Vahl)
The leban plant (Vitex pubescens Vahl) is a medicinal plant from the Vitex genus and the Lamiaceae family, a plant from the Vitex genus with the widest distribution in Indonesia. Leban contains several groups of bioactive compounds such as flavonoids, saponins, alkaloids and tannins, making Leban plants have potential as a source of antibacterial compounds. Endophytic fungi are mitosporic and meiosporic microorganisms that spend most of their lives in plant tissue. Endophytic fungi imitate the production of secondary metabolites in host plants. This research aims to isolate endophytic fungi from the leaves and bark of Leban plants and carry out antibacterial assay of endophytic fungal isolates using the well diffusion method. From the isolation results, 4 isolates were obtained from leaves and 4 isolates from stem bark. From these 8 isolates, only 6 isolates of endophytic fungi showed antibacterial activity. A total of 4 isolates of endophytic fungi (P1D1F1, P1D2F2, P1D3F1, and P1KB1F1) showed the ability to inhibit the growth of V. parahaemolyticus. A total of 2 isolates of endophytic fungi (P1D3F1 and P2KB3F1) were able to inhibit growth S. aureus. A total of 2 isolates of endophytic fungi (P1D3F1 and P3KB2F1) could inhibit the growth of
L. monocytogenes. Isolate P1D3F1 showed the best antibacterial potential with a broad spectrum against Gram-negative bacteria (L. monocytogenes) and Gram- positive bacteria (V. parahaemolyticus and S. aureus) so they can be used as candidates for producing antibacterial compounds. The results of this research show the potential of endophytic fungi from the leaves and bark of Leban plants as an alternative source of antibacterial compounds.
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