CD Skripsi

Isolasi Dan Uji Aktivitas Antimikroba Ekstrak Etil Asetat Dari Bonggol Nanas Ananas Comosus L

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Pineapple plants are crops that grow well in Indonesia. One part of the pineapple plant, namely the stem, contains active compounds, one of which is the enzyme bromelain, which functions as an antimicrobial. This research aims to isolate secondary metabolite compounds with antimicrobial activity from ethyl acetate extract. The sample was processed using the maceration method with ethyl acetate as the solvent. Separation of the ethyl acetate extract was performed using Vacuum Liquid Chromatography (VLC), which resulted in four fractions. Fractions 2 and 3 underwent further separation using gravity column chromatography, resulting in five fractions. In Fraction 2, the ethyl acetate extract yielded a compound coded Ac-EA, which is a white crystalline substance with a melting point of 120-122ºC. This crystal was characterized using FTIR spectroscopy. FTIR spectroscopy shows a wide absorption at around 3100 cm-1 (- OH); absorption in 2933 cm-1 (C-H) alifatik; 1370 cm- 1 (C-H3), 1454 cm-1 (CH2), and 1024 cm-1 (C-O). Phytochemical testing showed that the secondary metabolite contained in the Ac-EA compound is a terpene-type compound. Antimicrobial activity testing was conducted against the pathogenic bacteria S. aureus and E. coli, as well as the pathogenic fungus C. albicans using the diffusion method. The antimicrobial activity test results indicated that the ethyl acetate extract and the Ac-EA compound were able to inhibit the growth of S. aureus bacteria, while no inhibition zones were observed against E. coli bacteria and C. albicans fungi.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1703113331

Penerbit

Pekanbaru : Universitas Riau FMIFA Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1703113331

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

mardiah

Versi lain/terkait

Lampiran Berkas

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