CD Skripsi
Pemberian Pakan Mengandung Tepung Daun Indigofera Terfermentasi Terhadap Perubahan Aktivitas Enzim Pencernaan Dan Jaringan Usus Ikan Baung (Hemibagrus Nemurus)
This research was conducted on Januari-Agustus 2023, at Fish Nutrition Laboratory of Fishery and Marine Faculty of Riau University. The aim of the research is to determine the effect of using Indigofera leaf meal (Indigofera zollingeriana) fermented with Rhizopus sp. on the digestive enzyme activity and intestinal histology of Hemibagrus nemurus. This study employed a Completely Randomized Design (CRD) consisting of four treatment levels with three replications. The treatments consisted of incorporating fermented Indigofera leaf meal into the feed at 0%, 10%, 20%, 30%, and 40% levels. Hemibagrus nemurus fingerlings used had an average weight of 1.24 ± 0.02 g and were stocked in cages at a density of 25 fish/m³. Thetest feed was administered at 15%of thebiomass weight with afeeding frequency of three times a day for 56 days. After 56 days of feeding, the fish intestines were collected for histological observation and digestive enzyme activity measurement. To assess feed digestibility, the fish were fed a diet containing 0.5% Cr2O3 as an inert marker. The test fish, weighing 1.24 ± 0.02 g, were placed in aquariums with a stocking density of 25 fish/m³. After feeding, feces were collected and dried to measure the Cr2O3 content. The results showed that the use of 10% fermented Indigofera leaf meal with Rhizopus sp. resulted in the best feed digestibility (61.24%), and the fish intestines exhibited villi elongation, and the appearance of goblet cells. Additionally, the use of 40% fermented Indigofera leaf meal yielded the best results for digestive enzyme activity, with protease activity at 0.0394 IU/mL, lipase activity at 0.0541 IU/mL, and amylase activity at 0.6161 IU/mL
Keyword : Hemibagrus nemurus, Indigofera zollingeriana, Intestinal Histology, Enzyme Acticity
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