CD Skripsi

"PROFIL METABOLOMIK PLASMA DARAH MENCIT JANTAN DEPRESI YANG DIINJEKSI EKSTRAK n-HEKSAN BIJI PALA (Myristica fragrans Houtt.) MENGGUNAKAN LC-HRMS"

---

Depression is a neuropsychiatric disorder that affects behavior, emotions, and
thoughts in a negative direction. Nutmeg seed extract has the potential to be an
antidepressant that has been tested in vivo and in silico. The results of in vivo tests
using the forced swim tests (FST) method of double-test animals showed that 100
mg/kg of nutmeg n-hexane extract (ENHP) was able to reduce the immobility
time of mice by 45,97%. However, the test carried out was only an initial
screening and was unable to molecularly identify metabolites that act as
biomarkers of depression. Therefore, this study was conducted with the aim of
profiling metabolites found in the blood plasma of mice and identifying potential
candidate biomarkers of depression through an untargeted metabolomic approach.
The blood plasma of positive control mice (+), negative control mice (-), and
depressed mice injected with 100 mg/kg of ENHP was analyzed using LC-HRMS.
Data were processed using MS-DIAL, filtered and matched with the human
metabolome database (HMDB). Furthermore, a literature review was conducted to
obtain potential metabolite candidates for depression biomarkers. The final stage
was a multivariate statistical analysis (PCA and heatmaps) using MetaboAnalyst
to see the separation and proximity of metabolites between the three treatment
data. The results of MS-DIAL identification found 18.799 metabolite candidates
and produced 18 metabolite candidates after the filtering process and confirmation
of metabolites identified through HMDB with high confidence. Based on the
results of the literature review, five metabolite candidates were found as
biomarkers of depression, namely sphingomyelin, ceramide, dihydroceramide,
lysophospatidylcholine and choline which come from the lipid metabolism
pathway. The results of the PCA analysis showed that the analyzed metabolites
were responsible for the separation between groups and based on the heatmap
analysis, it was shown that the metabolite levels of the ENHP group had a close
relationship with the control group (+) compared to (-). So it can be concluded
that ENHP 100 mg/kg has the potential to be an antidepressant drug which can be
shown by the metabolite profile in depressed mice approaching normal
conditions.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

2003113757

Penerbit

Pekanbaru : . Universitas Riau – FMIPA – Kimia., 2024

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

2003113757

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

GUNTUR

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar