CD Skripsi
Uji Aktivitas Antimikroba Ekstrak Air Dan Fraksi Gabungan Dari Kulit Buah Semangka (Citrullus Vulgaris Schard)
SUMMARY
Watermelon (Citrullus vulgaris) is a fruit that contains a lot of nutrients such as protein, fat, vitamin A, vitamin B, vitamin C, iron, phosphorus and some amino acids that are beneficial for health. Watermelon tend to be consumed at the flesh of the fruit, while the white or skin thrown away so it is necessary to use the fruit skin. The purpose of this research is knowing antimicrobial activity and the results of fractionation extract from the rind Citrullus vulgaris (yellow). Dry samples were isolated by maceration method using aqua DM solvent that has been heated to a temperature of 60 ° C which produce viscous extract as much as 76.45 g. Viscous extract was separated by column chromatography which resulted in 5 fractions. One fraction (F3) fractionation results of analysis using IR spectroscopy which showed the presence of aliphatic C-H bond, C=O and C-O. Antimicrobial assay performed by the agar diffusion method against Gram-negative bacteria (Escherichia coli) and Gram-positive (Staphylococcus aureus) along with against the fungus Candida albicans. Antimicrobial assay viscous extracts and fractions showed activity in inhibiting the growth of both bacteria, but not for the growth of Candida albicans fungus. The best fractions which have inhibitory region (DDH) for Gram-negative bacteria (Escherichia coli) is a fraction F2 (10 mm) while for Gram-positive bacteria (Staphylococcus aureus) is a fraction F3 (8 mm).
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