CD Skripsi
Uji Aktivitas Senyawa Golongan Kumarin Dari Ekstrak Etil Asetat Daun Suren (Toona sureni (Blume) Merr)
ABSTRACT
Suren plants (Toona sureni (Blume) Merr) have several compounds which play a role as growth inhibitors, insecticides and antifeedants (inhibit the ability to eat) of insects. The isolation process of suren plants was done by maceration with water solvent and fractionated with ethylacetate solvent. The results of extraction obtained a crude extract of black ethylacetate as much as 17.07 grams. Ethylacetate extract was separated using VLC and obtained 7 fractions. Fraction 2 was recrystallized until 170 mg of orange color was labeled SEUTAG2. Cumar type positive SEUTAG crystals according to the test showing the blue color of the reagent identification test. Furthermore, the pure compound SEUTAG2 was tested for biological activity in the form of antioxidant test, toxicity test, antimicrobial test and insecticide test. The results obtained from each test were antioxidant activity of 3.52 ppm, anti-wing activity with LC50 value of 4,03%, toxicity activity obtaining an LC50 value of 2900 ppm and from the results of antimicrobial tests on bacteria and fungi, the pure compound SEUTAG2 was able to inhibit growth of E. coli and S. aureus bacteria had inhibitory dimeters of 14 mm and 14 mm of inhibition were also able to growth of C. albicans with a diameter of 10 mm at a concentration of 5.7 mg / mL with agar diffusion method.
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