CD Skripsi
Produksi Eksopolisakarida Oleh Bakteri Yang Diisolasi Dari Rhizosfer Padi Ladang
Exopolysaccharide is the result of microorganism activity and is found outside the cell wall. Exopolysaccharide has the potential to increase soil aggregates, produce growth hormones such as indole acetic acid and able to prevent drought. This study aimed to determine the exopolysaccharide production from isolates EPS1, EPS2, EPS3, EPS4, EPS5, EPS6, EPS7 and EPS8 based on various medium (nutrient broth, ATCC no.14, yeast extract mannitol broth) and pH (4,5,6) at room temperature for three days. The results of the physiological and biochemical characterization of eight isolates were negative Gram in the form of bacilli and cocci cells, positive catalase and fermenting carbohydrates with carbon sources of glucose, fructose and sucrose. The production of exopolysaccharide from the highest to the lowest were; EPS1 was 41.6 mg/mL using YEMB medium at pH 7, EPS2 was 22.53 mg/mL using ATCC no.14 at pH 7, EPS3 was 8.87 mg/mL using ATCC no.14 at pH 14 6, EPS4 was 19.23 mg/mL using ATCC medium no. 14 at pH 7, EPS5 was 33.9 mg/mL using NB medium at pH 7, EPS6 was 27.4 mg/mL using ATCC medium no. 14 at pH 4, EPS7 was 23.73 mg/mL using NB medium at pH 5 and EPS8 was 24.3 mg/mL using the YEMB medium at pH 5. The eight exopolysaccharide-producing bacteria can be developed as biofertilizer agents for upland rice plants in peat areas.
Keywords: Exopolysaccharide, nutrient broth, ATCC no.14, YEMB, pH
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