CD Tesis

Fraksinasi Kromatografi Kolom Fasa Terbalik Ekstrak Polar Senyawa Antioksidan Kulit Manggis (Garcinia Mangostana Linn)

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The crude polar extract of mangosteen pericarp not only has a relatively high antioxidant activity (55±4) μg/mL, but also has high cytotoxicity (16±0.5 μg/mL). This high cytotoxicity presumably is caused by the presence of complex cytotoxic compounds from the mangosteen pericarp. To obtain a non-toxic extract preparation with high antioxidant activity, crude polar 50% ethanol extracts of mangosteen pericarp were partially purified using reverse-phase column chromatography with Silica C18 as the stationary phase and an water-acetonitrile gradient elution. Fractions of 25 ml each were collected. From the 10 collected fractions, 6 fractions had high antioxidant activities, with IC50 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging antioxidant levels below 50 μg/mL. Three fractions (fractions 3, 5, and 7) with the highest antioxidant activities of (16.4±0.6) μg/mL, (17.8±2) μg/mL and (17.4±1.8) μg/mL respectively, were chosen for further cytotoxicity, phenolic content and High Performance Liquid Chromatography (HPLC) analysis. Cytotoxic tests were done with the Brine Shrimp Lethality Assay. Fraction 3 had low cytotoxicity (LC50 485±96 μg/mL) and fraction 5 was non-toxic (LC50 ≥ 1000 μg/mL), while fraction 7 still had high cytotoxicity (LC50 2.8±0.8 μg/mL). High Performance Liquid Chromatoraphy chromatogram profiles showed that fraction 3 and 5 contained more polar compounds than the compounds present in fraction 7. It can be concluded that the reverse phase method enabled isolation of a non-toxic fraction, that is fraction 5, with an higher antioxidant activity than in the crude original polar extracts.
Keywords: Garcinia mangostana, C18 reverse phase chromatography, IC50 2,2-diphenyl-1 picrylhydrazyl radical scavenging, Brine Shrimp Lethality Assay (BSLA).

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 05. 220 (0018)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia., 2020

Deskripsi Fisik

xii, 95 hlm,; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 05. 220 (0018)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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