CD Tesis

Keanekaragaman Genetik Geronggang (Cratoxylum Arborescens) Asal Riau Berdasarkan Marka Molekuler Random Amplified Polymorphic Dna (RAPD) Untuk Restorasi Gambut

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Geronggang (Cratoxylum arborescens) is a type of pioneer plant native to peatlands. Geronggang populations are becoming increasingly difficult to find in peat ecosystems due to the impact of logging, fire, and land conversion. The success of peatland rehabilitation through revegetation is influenced by the selection of plant species. Genetic diversity is an important factor in conservation efforts. An analysis of the diversity of geronggang trees from Riau has not been reported. The research aimed to determine the genetic diversity within and between three geronggang populations from Riau. Sampling was carried out in November 2019-February 2020 and laboratory analysis in July 2020. Leaf samples were analyzed at the Genetics Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Riau University. The three populations analyzed included from Bantan Timur Village (Bengkalis Regency), Rawa Mekar Jaya Village (Siak Regency) and Lukun Village (Meranti Islands Regency). The DNA isolation method using the manufacturer's instructions (Geneaid). Primary selection was carried out on 36 DNA samples in 10 primers. Each sample was amplified with 25 μl as standard volume in one reaction. The steps of the PCR process were pre-PCR at 94ºC for 5 minutes followed by 3 repeated steps of 35 cycles namely: denaturation at 94ºC for 45 seconds, annealing at 34ºC for 60 seconds, and extension at 72ºC for 90 seconds. The PCR process was ended with post PCR at 72 ºC for 10 minutes. The PCR amplification results were electrophoresed on 1.2% agarose gel (0.6 g agarose powder, 50 ml 1x TBE buffer (Tris-Borate-EDTA pH 8.0), and 2 μl Ethidium Bromide) in 1x TBE buffer. Electrophoresis was operated at 50 volts for 60 minutes. Primers selection was carried out on 10 primers from 36 geronggang individuals.
The results of the selection obtained 5 selected primers capable of amplifying 23 individuals of Crotoxylum arborescent namely OPD20, OPAD10, OPC19, OPZ10, and OPV10. The total bands produced were 91 with 83 (91.21%) polymorphic DNA bands with ranges of about 100 pb-2,500 pb. The genetic distance coefficient is 0.502 groups of geronggang individuals divided into 3 groups that have the same genetic kinship. The first group with genetic 0.470 consisted of 9 individuals and the second group consisted of 13 individuals, while the third group consisted of one individual. Genetic diversity (He) in geronggang population ranging from 0.1968 ± 0.2024 to 0.2321 ± 0.1895 with an average genetic diversity valued at 0.2178 ± 0.1839. The heterozygosity (He) value of geronggang population is moderate. The genetic difference between geronggang populations is moderate with a GST value of 0.149. The amount of genetic distance between populations was 0.0593 - 0.0976 and the Mekar Jaya and Bantan Timur populations have a genetic distance of 0.0976. The population of Rawa Mekar Jaya (Siak) village is a source of seeds recommendation for the peatland restoration program in Bengkalis regency.
Keywords: Geronggang, Genetic diversity, RAPD

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 10. 220 (0018)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Ilmu Pertanian., 2020

Deskripsi Fisik

xi, 49 hlm,; ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 10. 220 (0018)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

ILMU PERTANIAN

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

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