CD Tesis

Pemurnian Dan Penentuan Struktur Senyawa Metabolit Sekunder Dari Endofit Fusarium Oxysporum Lbkurcc41 Umbi Dahlia (Dahlia Variabilis)

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Bioactive compounds as drugs from microorganisms in today's era are needed, because the growth and production of the desired compounds from microorganisms can be controlled. This study aims to obtain antimicrobial bioactive compounds by isolating and purifying the secondary metabolite ethyl acetate extract of Fusarium LBKURCC41 isolates from fermentation using Huang's media by changing the carbon source to corn. Fusarium LBKURCC41 is an endophyte isolated from the tuber of Dahlia variabilis collected in Padang Luar, West Sumatra and it has been tested for its secondary metabolites as antimicrobials. The secondary metabolite extract was obtained from fermentation using Huang media. Secondary metabolite extracts were also obtained from Huang media, but the carbon sources were replaced with corn, sweet potatoes, and potatoes. The largest inhibition results were obtained from media using corn, which was able to inhibit S. aureus, C. albicans, and E. coli with the inhibition zone of each pathogen respectively 15.24 mm; 7,62 mm; and 14,15 mm. The separation and purification methods used TLC, fast chromatography, and recrystallization processes. Eluents with optimal separation were using butanol: acetic acid: water (BAW) with a ratio of 4: 1: 5. From the results of separation and purification, five compounds were obtained with the code BAW1, BAW2, BAW3, BAW4, and BAW5. The five compounds were tested for their antimicrobial activity against E. coli, S. aureus, and C. albicans using the paper disc diffusion method. Among the five compounds that have the highest activity is the BAW4 compound, then its structure is elucidated using UV, FT-IR, and NMR spectroscopy. After structural elucidation, the BAW4 compound was thought to be similar to a sesquiterpene compound called cyclonerodiol.
Keywords: antimicrobe, endophyte, Fusarium oxysporum, secondary metabolite

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

10 05. 221 (0001)

Penerbit

Pekanbaru : Universitas Riau – Pascasarjana – Tesis Kimia., 2021

Deskripsi Fisik

xiii, 78 hlm.: ill.: 29 cm

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

10 05. 221 (0001)

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

FATAH

Versi lain/terkait

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