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Isolasi Dan Identifikasi Bakteri Penghasil Biosurfaktan Asal Limbah Cair Rumah Potong Hewan (Rph)

ABSTRACT
The slaughterhouse wastewater is rich of oil and fat and it may inhabit by
biosurfactant producing bacteria. Isolation of the bacteria is needed in order to
find out a biosurfactant producing bacteria that can be used to maintain the oil and
fat polluted area through bioremediation process. To isolate and identify the
biosurfactant-producing bacteria, a study has been conducted on July to
September 2020. The samples were obtained from slaughterhouse wastewater at
Cipta Karya street Pekanbaru, Riau Province and samplings were conducted three
times. The isolation was conducted by TSB (Tryptic Soy Broth) and incubated
for 24 hours. Then the bacterial samples were planted by TSA (Tryptic Soy Agar)
and isolated for 24 hours. Then, it was identified using biochemical and
morphological tests were carried out to find out the types of the bacteria. The
Emulsification Index was then calculated by using TSB (Tryptic Soy Broth) and
kerosene. Results shown that the emulsification index were Flavobacterium 53%,
Agrobacterium 53%, Serratia 60%, Salmonella 52, 5%, Salmonella 52.4%,
Proteus 65.7%, Clostridium 62% and Aeromonas 59.5%. As Proteus shown the
highest Emulsification Index, it was check using a PCR (Polymerase Chain
Reaction) and it is proved that the ecies is Proteus vulgaris.
Keywords : Proteus vulgaris, Emulsification Index, slaughterhouse liquid waste,
bacteria isolation

Ketersediaan
#
Perpustakaan Universitas Riau 04 01. 121 (0019)
04 01. 121 (0019)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
04 01. 121 (0019)
Penerbit
Pekanbaru : Universitas Riau – Fakultas Perikanan Dan Ilmu Kelautan - Manajemen Sumberdaya Perairan.,
Deskripsi Fisik
xii, 29 hlm.; ill.: 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
04 01. 121 (0019)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
MANAJEMEN SUMBERDAYA PERAIRAN
Info Detail Spesifik
-
Pernyataan Tanggungjawab
Versi lain/terkait

Tidak tersedia versi lain

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