CD Skripsi
Isolasi Dan Identifikasi Bakteri Penghasil Biosurfaktan Asal Limbah Cair Tahu Ud. Dika Putra, Provinsi Riau
Abstract
Tofu liquid waste contains high levels of organic matter, especially protein and amino acids. These organic compounds can be proteins, carbohydrates and fats. Most of the bacteria are able to use oil or fat as a source of carbon and energy, bacteria that have this ability are often known as lipolytic bacteria. This study aims to obtain biosurfactant producing bacteria from tofu wastewater.This research was conducted from July - September 2020. The method used in this research is survey method and emulsification method. The media used for bacterial isolation were Tryptic Soy Broth (TSB) and Tryptic Soy Agar (TSA). 6 of the isolates are able to produce biosurfactants. Morphological and biochemical characteristics indicate six bacterial genera, namely Genus Agrobacterium sp. with an emulsification index of 53%, Genus Proteus sp. with an emulsification index of 45%, Genus Citrobacter sp. with an emulsification index of 59%, Genus Enterobacter sp. with an emulsification index of 62%, and Genus Serratia sp. with an emulsification index of 50%. The DNA identification of bacterial isolates that had the highest emulsification index was Proteus mirabilis with an emulsification index of 64%. This shows that Proteus mirabilis bacteria have the potential to degrade oil in a polluted environment.
Keywords: Bacteria, Biosurfactant, Emulsification, Tofu Liquid Waste, Proteus mirabilis.
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