CD Skripsi

Isolasi Dan Uji Antibakteri Senyawa Metabolit Sekunder Ekstrak Etil Asetat Dari Daun Tumbuhan Akar Kuning (Arcangelisia Flava (L.) Merr)

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Arcangelisia flava (L.) Merr, commonly known as "akar kuning," is a member of the Menispermaceae family that grows in Indonesia’s tropical forests and is characterized by its yellow climbing stem. Traditionally, this plant has been used to treat malaria, diabetes, and skin infections. This study aims to isolate secondary metabolite compounds from the ethyl acetate extract of A. flava leaves and evaluate their antibacterial activity using the microdilution method against Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium, and Vibrio parahaemolyticus. The leaves were extracted through successive maceration using solvents of increasing polarity in the following order: n-hexane, dichloromethane, ethyl acetate, and methanol. The ethyl acetate extract was further separated using vacuum liquid chromatography (VLC), yielding six main fractions. The second fraction was further purified by flash column chromatography, resulting in seven subfractions. The second subfraction (SF2) was recrystallized and showed a single spot under anisaldehyde staining on TLC plates. This compound, labeled as 24-AK-EA-FII- SF2, had a melting point in the range of 185–187°C, indicating that the compound was pure. The compound was identified as stigmasterol acetate based on steroid testing, TLC comparison, and FTIR analysis with standard compounds. Antibacterial testing revealed that stigmasterol acetate exhibited inhibitory activity with Minimum Inhibitory Concentration (MIC) of 100 ppm against S. aureus and
B. cereus, and 125 ppm against S. typhimurium and V. parahaemolyticus. The Minimum Bactericidal Concentration (MBC) values were higher than the MIC, exceeding 200 ppm for all tested bacteria. These findings indicate that A. flava leaves have potential as a natural antibacterial agent.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2103110608

Penerbit

Pekanbaru : ., 2025

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2103110608

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

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