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Isolasi Dan Uji Aktivitas Bakteri Heterotrofik Terhadap Bakteri Patogen Dari Perairan Laut Kabupaten Siak, Provinsi Riau

Heterotrophic bacteria are bacteria that require organic carbon and inorganic nitrogen as a source of nutrition. This research aimed to determine the number of heterotrophic bacteria, their activity analysis, against pathogenic bacteria (Vibrio sp, Aeromonas sp, Pseudomonas sp) and to identify the species of heterotrophic bacteria through DNA analysis isolated from estuary waters of 15 ppt of in the industrial estuary at coastal areas Sungai Kayu Ara village with salinity 27 ppt, Siak District. Distribution of bacteria at station 1 was higher than station 2 with value of nilai 7,04 x 107 cfu / ml at station 1 and 6,46 x 107cfu / ml at station 2. Based on the antagonism test against pathogenic bacteria, it was found that there were 9 bacterial isolates that potentially inhibited the growth of the three pathogenic bacteria, namely: RM3, RM4, RM5, RM6, RM8, RM11, RL15, RL17 and RL 24 with inhibit zone ranges from 0.6 - 18mm, for the RM11 (12-15.3 mm) isolate was the best isolate in its inhibitory properties. By DNA test with 16S rRNA method and BLAST analysis, the isolates were identified as Bacillus safensis, Alcaligenes faecalis, Enterobacter sp, Enterococcus sp and Vagococcus sp. In identified there were two unidentified isolates , that were possibly non-registered species in Genbank NCBI because it has a homology percentage of only 84%.
Keywords: Heterotrophic Bacteria, Antagonism, DNA, Analysis, Estuary, 16SrRNA

Ketersediaan
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Perpustakaan Universitas Riau 04 03. 118 (0011)
04 03. 118 (0011)
Tersedia
Informasi Detail
Judul Seri
-
No. Panggil
04 03. 118 (0011)
Penerbit
Pekanbaru : Universitas Riau – Fakultas Perikanan Dan Kelutan - Ilmu Kelautan.,
Deskripsi Fisik
xvii, 103 hlm.; ill.; 29 cm
Bahasa
Indonesia
ISBN/ISSN
-
Klasifikasi
04 03. 118 (0011)
Tipe Isi
-
Tipe Media
-
Tipe Pembawa
-
Edisi
-
Subjek
ILMU KELAUTAN
Info Detail Spesifik
-
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