CD Skripsi
Uji Biodegradasi Pet (Polyethylene Terephthalate) Oleh Bakteri Termofilik Yang Diisolasi Dari Sumber Air Panas Bukik Gadang, Solok, Sumatera Barat
Plastic is a synthetic polymer compound formed from the polymerization of monomers composed of hydrocarbon bonds. The high use of plastic, including for household purposes, impacts increasing piles of plastic waste, one of the efforts made to reduce this problem is to carry out biodegradation. This study aimed to determine the ability of thermophilic bacteria with isolate codes LBKURCC185, LBKURCC186, LBKURCC187, LBKURCC188, LBKURCC189, and LBKURCC190 genus Pseudomonas sp. and Thermus sp. to degrade Polyethylene Terephthalate (PET) plastic during an incubation period of 4 weeks using a temperature of 50 oC. This research method is a biodegradation test in Nutrient Agar (NA) and Mineral Salt Medium (MSM) solid media with plastic loss weight determination, FTIR analysis, and SEM analysis. The results showed that all bacterial isolates could degrade PET which was characterized by a decrease in PET weight, changes in functional groups and wave numbers from the FTIR results, and an uneven PET surface from SEM analysis. The bacteria with the highest percentage of degrading PET were the LBKURCC185 bacterial isolate of the Thermus sp. and LBKURCC186 genus Pseudomonas sp. which experienced a decrease in PET weight of 0.85% in the MSM test medium. LBKURCC185 bacteria decreased PET weight by 0.4% and LBKURCC186 by 0.62% in NA test media. FTIR results showed a decrease in wave number, bond breaking, and formation of functional groups at O-H, C-H aliphatic, C=O, C=C, and C-O; as well as changes in the percentage value of the transmission. SEM analysis showed the formation of damage to the surface of the PET test with the addition of LBKURCC186 bacteria which made the PET surface uneven, there were cavities and lumps after comparison with the control PET whose surface looked smooth after an incubation period of 4 weeks.
Tidak tersedia versi lain