CD Skripsi

nalisis Sekuens Barkode Dna Pada Gen Matk Tumbuhan Maman Asal Riau

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ABSTRACT
DNA barkoding is a sequence of short DNA sequences that have been standardized to make it easier to identify a species quickly, precisely and accurately. This study used DNA barcodes on the matK gene to analyze the DNA barcode sequences of maman plants from Riau. The stages of the research were sampling, morphological observation, total DNA isolation, total DNA electrophoresis, PCR using the Genaid Mini Plant kit, electrophoresis of PCR products, sequencing and data analysis using the software BioEdit, BLASTn and MEGA version 11. The results of the morphological observations showed that Maman plants have upright stems purplish green to brownish green, white flower petals with 4 petals, five finger leaves, fruit shaped like upright capsules with green pointed ends, when ripe maman fruit will be yellow-brown, seeds The maman fruit is blackish brown in color, circular and flat in shape, when the fruit is ripe the seeds will turn black. The length of the sequences obtained from the three samples has a length of 754 bp. The results of the BLASTn analysis found similarities or identity values that reached 100%. The highest identity value was found in Cleome gynandra by 100.00%, and the lowest value was found in Dracocephalum heterophyllum by 91.63%. There were 73 nucleotide variations among the studied accessions and 12 of them were critical nucleotides.
Keywords: BLASTn, DNA Barkoding, Maman, matK, Polymerase Chain Reaction.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

1903113106

Penerbit

Pekanbaru : Universitas Riau - FMIPA -BIOLOGI., 2023

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

1903113106

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

BIOLOGI

Info Detail Spesifik

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Pernyataan Tanggungjawab

tethi

Versi lain/terkait

Lampiran Berkas

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