CD Skripsi

Isolasi Dan Uji Aktivitas Antibakterisenyawa Metaboli Sekunder Dari Ekstrak Diklorometana Tumbuhan Akar Kering ( Arcangelisia Flafa )

---

SUMMARY
Yellow root (Arcangelisia flava) is a liana plant that belongs to the family of Menispermaceae. The extracts from various parts from this yellow root also show several pharmacological activities that have been studied, including as antimalarial, anticancer, antioxidant, antifungal, antidiabetic, antidepressant, even as an antibacterial. The study aimed to isolate secondary metabolite compounds of dichloromethane extract from A. flava plants and assess their antibacterial activity. Secondary metabolites was obtained from maceration using methanol solvent. The crude methanol maserate was carried out by liquid-liquid extraction using n-hexane, dichloromethane, and ethyl acetate solvents. The dichloromethane extract was separated using the vacuum liquid chromatography (VLC) method so that 11 fractions and 4 bands were obtained. Then thin-layer chromatography (TLC) was carried out to see the same stain, so that 9 main fractions were obtained. In fraction 6 there was a yellow precipitate, and then was purified with recrystallization. The TLC test for fraction 6 yielded one spot visible with a 366 and 254 nm UV lamps. The purity was also verified by HPLC and melting point tests. The melting point test yielded a value between 204-206oC and HPLC results obtained one peak, this indicates that the compound was pure. The pure compound was coded 22-10-B-FVI and then UV-Vis, FTIR and NMR characterization was carried out. The results of UV-Vis characterization of the compound 22-10-B-FVI that revealed peaks at wavelength 226 with an absorbance value of 0.387, at a wavelength of 268 nm with an absorbance value of 0.370, at a wavelength of 342 nm with an absorbance value of 0.391 and peaks at 431 nm with an absorbance value of 0.090. FTIR characterization revealed the presence of aliphatic and aromatic of C-H, C-N, alkenes and aromatics of C=C and C-O groups. The results of this characterization showed that the compound 22-10-B-FVI was belongs to the group of alkaloids, namely palmatine. This was proved by NMR analysis data. Antibacterial activity tests were performed on Gram-positive and Gram-negative bacteria. The compound 22-10-B-FVI had a MIC value of 25 μg/mL for S. aureus ATCC 6538, L. monocytogenes ATCC 7644, V. alginolyticus ATCC 17749 and B. subtilis ATCC 6633 bacteria and a MIC value of 100 μg/mL for S. Typhimurium ATCC 14028 bacteria. It had MBC values of 25 μg/mL for V. alginolyticus ATCC 17749, 50 μg/mL for S. aureus ATCC 6538 and 100 μg/mL for L. monocytogenes ATCC 7644 and B. subtilis ATCC 6633.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

1903112007

Penerbit

Pekanbaru : Universitas Riau - FMIPA - Kimia., 2023

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

1903112007

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

TETHI

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar