CD Skripsi

Deteksi Variasi Genetik Gen E6 Pada Human Papillomavirus Tipe 16

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Cervical cancer, caused by high-risk Human Papillomavirus (HPV) infection with 16 and 18 being the most common type, ranks second highest in mortality with 57% fatality rate in Indonesia. The E6 gene in HPV-16 has an oncogenic nature which transforms HPV-16 infections into cervical cancers. By its geographical area, E6 gene mutation generates genetic variation of HPV-16 which significantly correlates with the progression of cervical cancer. This study aims to present the genetic variation of the HPV-16 E6 gene in Riau Province, Indonesia. A total of 37 HPV-detected isolates using MY09/MY11 primer was then followed by the HPV-16 E6 gene amplification using specific primers and sequencing process. All 7 HPV-16 E6 sequences obtained were then processed using BioEdit, aligned with NC_001526.4 as well as reference sequences from respective lineage, and analyzed using a phylogenetic tree. Results show 19 HPV-16 infections, 13 of which are co-infections with HPV-18. From 7 samples with a total of 13 point mutations, 4 (30,7%) 7318A>G was found to be the most common mutation which alters the amino acid sequence 65N>S. Phylogenetic analysis shows specimens 79, 83, 87, 94, and 104 fall in the A lineage, with 79, 83, 87 in the A3 sublineage (European) and 94, 104 in the A1 sublineage (European), while specimen 89 falls in the C lineage (African-2). In conclusion, 7318A>G is the most common genetic variation found in HPV-16-infected cervical cancer patients in Riau Province.
Keywords: Genetic variation, HPV type 16, E6 gene, phylogenetic.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2008135279

Penerbit

Pekanbaru : Universitas Riau FK Pendidikan Dokter., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2008135279

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN DOKTER

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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