CD Tesis

Isolasi Dan Uji Aktivitas Antioksidan Ekstrak Diklorometana Pyrrosia Lanceolata

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The epiphytic fern Pyrrosia lanceolata is used as a folk medicine and is widely recognized for its efficacy by the general public. Isolating secondary metabolites and determining their activity are just two of the many successful studies that have been conducted. This research was conducted with the objectives of isolating and characterizing the pure compounds that obtained from the dichloromethane extract of the epiphytic fern P. lanceolata, as well as determining the level of antioxidant activity possessed by the various extracts, fractions, and pure compounds. Maceration with metanol was used to extract P. lanceolata in this study, and the resulting compound was then partitioned with n-hexane, followed by dichloromethane, and finally ethyl acetate. The dichloromethane extract was then subjected to a separation process involving vacuum liquid chromatography (VLC), which resulted in the production of five primary fractions (F1, F2, F3, F4 and F5). The main fraction was tested for antioxidant activity and the best IC50 was obtained for F2. After employing flash column chromatography on F2 for the separation process, 210 vials were collected and subsequently combined into 12 primary subfractions based on TLC assay result. SF5 generates a pure compound that is yellowish white in color and has a melting point that ranges from 256-258℃. It was code is 21-08-B-FII-SFV-71-90/210. A pure yellow compound with a melting point of 224-226℃ was produced in SSF1 after SF6 was separated using gravity column chromatography. The compound's code was 21-08-B-FII-SFVI-SSFI-1-20/303, and its color was yellow. The UV-Vis, FTIR, and NMR spectrophotometers were used in order to elucidate the structure of the compounds. In accordance with the findings of the spectroscopy, the compound 21-08-B-FII-SFV-71-90/210 was identified as apigenin, whereas compound 21-08-B-FII-SFVI-SSFI-1-20/303 as kaempferol. The results of the antioxidant activity assay using the DPPH method showed that the two compounds had IC50 values of >1000 and 17 ppm , respectively.
Keywords : Pyrrosia lanceolata, antioxidant, DPPH.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

2110246987

Penerbit

Pekanbaru : Universitas Riau Pascasarjana Tesis Pendidikan Kimia., 2024

Deskripsi Fisik

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Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

2110246987

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

PENDIDIKAN KIMIA

Info Detail Spesifik

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Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

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