CD Tesis

Isolasi Senyawa Metabolit Sekunder Dan Uji Aktivitas Antibakteri Dan Antijamur Dari Ekstrak Etil Asetat Kulit Batang Tumbuhan Medang Lendir (Litsea Glutinosa)

---

Medang lendir (Litsea glutinosa), a plant extensively utilized in traditional medicinal practices, offers valuable leaf extracts that serve as both a pain alleviator and a mild astringent, effectively treating conditions like diarrhea and dysentery. Numerous studies have underscored its significance by isolating and analyzing its secondary metabolites for bioactivity. The focus of this research is twofold: firstly, to isolate and thoroughly characterize pure compounds derived from the ethyl acetate extract of L. glutinosa, and secondly, to assess their antibacterial and antifungal efficacy through rigorous bioactivity testing on the extracts, fractions, and isolated compounds.
The methodological approach involved macerating the stem bark of L. glutinosa using methanol, followed by a meticulous partitioning with n-hexane and ethyl acetate. Subsequent separation through vacuum liquid chromatography (VLC) yielded 12 principal fractions. From these, Fraction 3 was selected for further refinement using gravity column chromatography, culminating in 30 vials that were consolidated into seven primary subfractions. Notably, subfraction SF7 yielded a pure compound of a yellowish-white hue, with a melting point ranging between 124-126°C. This compound underwent extensive characterization employing techniques such as UV-Vis spectrophotometry, FTIR, and NMR. These analyses revealed the compound to be conocarpan, a complex molecule with potential therapeutic applications. The compound's bioactivity was rigorously tested using the microdilution method, which indicated its significant antibacterial properties, demonstrated by the lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 12.5 μg/mL against B. subtilis. Moreover, it exhibited notable antifungal activity, with the lowest MIC and minimum fungicidal concentration (MFC) values of 12.5 μg/mL and 25 μg/mL, respectively, against C. glabrata and C. krusei. This research not only contributes to the understanding of L. glutinosa's pharmacological potentials but also paves the way for future studies to explore and harness the full spectrum of bioactive compounds within this plant for therapeutic applications. The findings underscore the intricate relationship between traditional knowledge and modern scientific approaches in uncovering new avenues for natural product-based drg discovery.
Key words : Litsea glutinosa, antibacterial, antifungal, microdillution.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

2110246861

Penerbit

Pekanbaru : Universitas Riau Pascasarjana Tesis Pendidikan Kimia., 2024

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

2110246861

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

PENDIDIKAN KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Jaka

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar