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Biosintesis Dan Karakterisasi Nanopartikel Zno Menggunakan Ekstrak Daun Ketapang (Terminalia Catappa) Dan Uji Sifat Antibakteri
Alternative approaches in biosynthesis have been applied to create ZnO nanoparticles with the aim of minimizing environmental impact. ZnO nanoparticles produced through the biosynthesis process show strong antibacterial properties, with high toxicity to bacteria but minimal impact on human cells. The aim of this research is to synthesize ZnO nanoparticles and analyze the impact of the volume ratio of precursor and reductant on the physical properties and antibacterial properties against Escherichia coli and Staphylococcus aureus bacteria. The synthesis process was carried out using varying volumes of ketapang leaf extract (Terminalia Catappa) and zinc nitrate hexahydrate (Zn(NO3)2.6H2O) solution, namely A (1:1), B (1:5), and C (1:10), followed by the addition of 1 M NaOH until each variation reached pH 7. Characterization of the nanoparticles using a UV-Vis Spectrophotometer showed absorption peaks at a wavelength range of 275-330 nm, with absorption intensities respectively 2.08; 1.74; and 1.248 a.u. The band gap energy value is in the range of 2.88-3.22 eV. XRD characterization of sample A showed a particle size of around 14.98 nm with a Miller index (100); (002); (101); (102); (110); (103); and (112). SEM analysis revealed a spherical morphology with an average size of 82.4 nm. FTIR examination showed that the ZnO nanoparticles resulting from biosynthesis using Ketapang leaf extract contained the functional groups O-H, C=C aromatic, C-H, C-N, and C-O. Tests of antibacterial properties against S. aureus and E. coli showed that ZnO nanoparticles with a test solution dilution concentration of 0.3 gr/ml, as well as observations for 24 hours, 48 hours and 72 hours, were effective as an antibacterial in inhibiting the growth of Escherichia coli bacteria. and Staphylococcus aureus.
Keywords: ZnO nanoparticles, biosynthesis, ketapang leaf extract, antibacterial properties, Escherichia coli, Staphylococcus aureus
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