CD Skripsi

Isolasi, Karakterisasi, Dan Identifikasi Bakteri Asam Laktat (Bal) Dari Cincalok Makanan Tradisional Kabupaten Indragiri Hilir

---

Cincalok, a traditional food from Indragiri Hilir Regency, is made by spontaneous fermentation of rebon shrimp (Acetes sp.) mixed with salt and sago. This food is thought to contain lactic acid bacteria (LAB) that have the potential as probiotic agents that can provide positive benefits to the body's health if consumed in the right amount. This study aimed to isolate LAB and characterize them macroscopically, microscopically, physiologically, and biochemically, as well as identify the genus of LAB that were successfully found. Isolation of lactic acid bacteria (LAB) was done through stratified dilution from 10-3 to 10-⁷, followed by inoculation using the spread plate method. A catalase test was performed to distinguish between isolates indicated as LAB and non-LAB. After that, the purification process of LAB isolates was carried out using the quadrant streak method, and their purity was carried out through macroscopic and microscopic testing. Furthermore, physiological and biochemical tests were carried out including CO₂ production tests, resistance to NaCl salt, temperature resistance, and resistance to pH to identify the genus of LAB contained in Cincalok. The results of bacterial isolation taken from Cincalok, a traditional food of Indragiri Hilir Regency, found 32 bacterial isolates, only 11 isolates showed catalase-negative results and indicated as lactic acid bacteria (LAB). Morphological characterization showed that 11 isolates had Gram-positive cells with round (cocci) or rod (bacilli) shapes. Based on physiological and biochemical tests, these isolates are homofermentative, able to grow in the pH range of 2 to 10, and tolerant of 4%, 6.5%, and 10% NaCl concentrations, as well as temperatures of 25°C and 45°C. Among these isolates, isolate CUR32 showed superiority due to its ability to survive in extreme conditions, making it a potential candidate as a probiotic agent. The identification results refer to Bergey's Manual of Systematic Bacteriology, Vol. 3, Edition 2, Firmicutes, of the 11 isolates, 3 genera were found that indicated as Lactobacillus spp., Enterococcus spp., and Streptococcus spp.

---

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

2003111265

Penerbit

Pekanbaru : Universitas Riau FMIPA Kimia., 2024

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

2003111265

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

-

Subjek

KIMIA

Info Detail Spesifik

-

Pernyataan Tanggungjawab

Mutia

Versi lain/terkait

Lampiran Berkas

Komentar

---

Anda harus masuk sebelum memberikan komentar